Mutation analysis of components of the Mediator kinase module in MED12 mutation-negative uterine leiomyomas

Br J Cancer. 2014 Apr 29;110(9):2246-9. doi: 10.1038/bjc.2014.138. Epub 2014 Mar 18.

Abstract

Background: Kinase module of Mediator complex ('CDK8 submodule') consists of four subunits: CDK8, Cyclin C, MED12, and MED13. Recently, we reported recurrent MED12 mutations in 70% of uterine leiomyomas. The aim of this study was to analyse whether mutations in other components of the module contribute to the development of these lesions.

Methods: Mutation screening of altogether 70 MED12 mutation-negative uterine leiomyomas was carried out by direct sequencing.

Results: None of the tumours displayed somatic mutations in the coding regions of CDK8/CDK19, CCNC, or MED13.

Conclusions: Mutations in CDK8/CDK19, CCNC, and MED13 do not frequently contribute to genesis of uterine leiomyomas.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cyclin C / genetics
  • Cyclin-Dependent Kinase 8 / genetics
  • Cyclin-Dependent Kinases / genetics
  • DNA Mutational Analysis
  • Female
  • Genetic Testing
  • Humans
  • Leiomyoma / genetics*
  • Mediator Complex / genetics*
  • Uterine Neoplasms / genetics*

Substances

  • CCNC protein, human
  • Cyclin C
  • MED12 protein, human
  • MED13 protein, human
  • Mediator Complex
  • CDK19 protein, human
  • CDK8 protein, human
  • Cyclin-Dependent Kinase 8
  • Cyclin-Dependent Kinases