Laboratory control of immunocytochemistry

Eur J Haematol. 1989 Jan;42(1):67-71. doi: 10.1111/j.1600-0609.1989.tb00249.x.


Monoclonal antibody technology is now used in many haematology departments for the analysis and phenotyping of haematological malignancies. Immunocytochemical analysis, in particular the alkaline phosphatase anti-alkaline phosphatase (APAAP) technique is increasing in popularity. This paper describes the findings from the 1st year of an external quality assurance scheme for immunocytochemistry. Initially, there was a wide variation amongst the results received. An improvement was seen over the year with subsequent concordance in the results. This variation could be due to a number of variables within the technique which this report highlights.

MeSH terms

  • Antibodies, Monoclonal
  • Antigens / analysis
  • Antigens, CD19
  • Antigens, Differentiation, B-Lymphocyte / analysis
  • HLA-DR Antigens / analysis
  • Humans
  • Immunohistochemistry / standards*
  • Laboratories / standards*
  • Quality Control


  • Antibodies, Monoclonal
  • Antigens
  • Antigens, CD19
  • Antigens, Differentiation, B-Lymphocyte
  • HLA-DR Antigens