Serum biomarker for diagnosis of endometriosis

J Cell Physiol. 2014 Nov;229(11):1731-5. doi: 10.1002/jcp.24620.


Endometriosis is estimated to affect 10% of women during the reproductive years. The lack of a non-invasive diagnostic test significantly contributes to the long delay between onset of the symptoms and definitive diagnosis of endometriosis. This case-control study was conducted to identify specific endometriosis antigens using 2D gel analysis in women with endometriosis (n = 5) and without endometriosis (n = 5). Differentially expresses spots were analyzed using matrix-assisted laser desorption/ionization-time-of-flight/mass spectrometry (nanoLC-ESI-MS/MS) with MASCOT analysis, in order to identify the corresponding proteins. ELISAs were performed on a different cohort of endometriosis (n = 120) and healthy patients (n = 20) in order to confirm the differential expression of the identified proteins. ROC analysis of ELISA results confirmed the statistical significance of the differential expression for one of these proteins: Zn-alpha2-glycoprotein (P = 0.019). We propose the analysis of the expression level of this protein in the serum as a new non-invasive diagnostic test for endometriosis.

MeSH terms

  • Adult
  • Biomarkers / blood*
  • Biomarkers / chemistry
  • Case-Control Studies
  • Electrophoresis, Gel, Two-Dimensional
  • Endometriosis / blood*
  • Endometriosis / diagnosis*
  • Female
  • Humans
  • Mass Spectrometry
  • Proteins / chemistry
  • Proteins / metabolism
  • ROC Curve
  • Reproducibility of Results
  • Silver Staining


  • Biomarkers
  • Proteins