Localization of substance P and neurofilament immunoreactive fibers in the lumbar facet joint capsule and supraspinous ligament of the rabbit

Brain Res. 1988 Sep 20;460(2):379-82. doi: 10.1016/0006-8993(88)90386-1.

Abstract

An indirect immunofluorescence method was utilized to identify substance P-like immunoreactive (SPLI) and neurofilament protein immunoreactive (NFIR) fibers in the lumbar facet joint capsule and supraspinous ligament of the rabbit. The results demonstrated a large population of NFIR fibers, indicating that these tissues are richly innervated, and a smaller population of SPLI fibers. In some fibers, neurofilament protein and substance P were colocalized. The data suggest that the facet joint capsule and the supraspinous ligament contain SPLI nociceptive fibers that could be a source of low back pain.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Cytoskeleton / ultrastructure*
  • Fluorescent Antibody Technique
  • Intermediate Filament Proteins / analysis*
  • Intermediate Filament Proteins / immunology
  • Intermediate Filaments / ultrastructure*
  • Joints / cytology*
  • Ligaments / cytology
  • Neurofilament Proteins
  • Rabbits
  • Spine / cytology
  • Substance P / analysis*
  • Substance P / immunology

Substances

  • Antibodies, Monoclonal
  • Intermediate Filament Proteins
  • Neurofilament Proteins
  • Substance P