Comparison of miR-124-3p and miR-16 for early diagnosis of hemorrhagic and ischemic stroke

Clin Chim Acta. 2014 Jun 10;433:139-44. doi: 10.1016/j.cca.2014.03.007. Epub 2014 Mar 17.


Background: The present study aimed to investigate and compare plasma concentrations of miR-124-3p and miR-16 as diagnostic markers in acute stroke.

Methods: miR-124-3p and miR-16 concentrations in 93 stroke patients were analyzed using real-time polymerase chain reaction. The primary outcome was the differentiation of hemorrhagic and ischemic stroke.

Results: Of 93 patients, 74 (79.6%) were diagnosed as ischemic stroke (IS) and 19 (20.4%) were diagnosed as hemorrhagic stroke (HS). Median plasma 124-3p concentrations taken within 24h of symptom onset were higher in HS patients than that in IS patients (3.8×10(5) vs 2.0×10(5) copies/ml plasma, p=0.0109), while median miR-16 concentration in IS patients were higher than that in HS patients (1.6×10(9) vs 1.3×10(9) copies/ml plasma, p=0.0399). The odds ratio (OR) for discriminating HS and IS with miR-124-3p and miR-16 was 5.37 and 9.75 respectively.

Conclusion: Both miR-124-3p and miR-16 are diagnostic markers to discriminate HS and IS.

Keywords: Diagnosis; Hemorrhagic stroke; Ischemic stroke; MicroRNA; Plasma.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Apoptosis
  • Biomarkers / blood
  • Brain / metabolism
  • Brain / pathology
  • Brain Ischemia / complications*
  • Early Diagnosis
  • Female
  • Humans
  • Intracranial Hemorrhages / complications*
  • Male
  • MicroRNAs / blood*
  • Middle Aged
  • Organ Specificity
  • Stroke / blood*
  • Stroke / complications
  • Stroke / diagnosis*
  • Stroke / pathology


  • Biomarkers
  • MIRN124 microRNA, human
  • MIRN16 microRNA, human
  • MicroRNAs