A novel perfused rotary bioreactor for cardiomyogenesis of embryonic stem cells

Biotechnol Lett. 2014 May;36(5):947-60. doi: 10.1007/s10529-014-1456-y. Epub 2014 Mar 21.


Developments in bioprocessing technology play an important role for overcoming challenges in cardiac tissue engineering. To this end, our laboratory has developed a novel rotary perfused bioreactor for supporting three-dimensional cardiac tissue engineering. The dynamic culture environments provided by our novel perfused rotary bioreactor and/or the high-aspect rotating vessel produced constructs with higher viability and significantly higher cell numbers (up to 4 × 10(5) cells/bead) than static tissue culture flasks. Furthermore, cells in the perfused rotary bioreactor showed earlier gene expressions of cardiac troponin-T, α- and β-myosin heavy chains with higher percentages of cardiac troponin-I-positive cells and better uniformity of sacromeric α-actinin expression. A dynamic and perfused environment, as provided by this bioreactor, provides a superior culture performance in cardiac differentiation for embryonic stem cells particularly for larger 3D constructs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bioreactors*
  • Cell Culture Techniques / instrumentation*
  • Cell Culture Techniques / methods
  • Cell Differentiation / physiology*
  • Embryonic Stem Cells / cytology*
  • Mice
  • Myocytes, Cardiac / cytology*