Evaluating the virucidal efficacy of hydrogen peroxide vapour

J Hosp Infect. 2014 Apr;86(4):255-9. doi: 10.1016/j.jhin.2014.02.003. Epub 2014 Feb 27.


Background: Surface contamination has been implicated in the transmission of certain viruses, and surface disinfection can be an effective measure to interrupt the spread of these agents.

Aim: To evaluate the in-vitro efficacy of hydrogen peroxide vapour (HPV), a vapour-phase disinfection method, for the inactivation of a number of structurally distinct viruses of importance in the healthcare, veterinary and public sectors. The viruses studied were: feline calicivirus (FCV, a norovirus surrogate); human adenovirus type 1; transmissible gastroenteritis coronavirus of pigs (TGEV, a severe acute respiratory syndrome coronavirus [SARS-CoV] surrogate); avian influenza virus (AIV); and swine influenza virus (SwIV).

Methods: The viruses were dried on stainless steel discs in 20- or 40-μL aliquots and exposed to HPV produced by a Clarus L generator (Bioquell, Horsham, PA, USA) in a 0.2-m(3) environmental chamber. Three vaporized volumes of hydrogen peroxide were tested in triplicate for each virus: 25, 27 and 33 mL.

Findings: No viable viruses were identified after HPV exposure at any of the vaporized volumes tested. HPV was virucidal (>4-log reduction) against FCV, adenovirus, TGEV and AIV at the lowest vaporized volume tested (25 mL). For SwIV, due to low virus titre on the control discs, >3.8-log reduction was shown for the 25-mL vaporized volume and >4-log reduction was shown for the 27-mL and 33-mL vaporized volumes.

Conclusion: HPV was virucidal for structurally distinct viruses dried on surfaces, suggesting that HPV can be considered for the disinfection of virus-contaminated surfaces.

Keywords: Adenovirus; Decontamination; Disinfection; Feline calicivirus; HPV; Hydrogen peroxide vapour; Influenza virus; Norovirus; SARS; Transmissible gastroenteritis virus.

MeSH terms

  • Disinfectants / pharmacology*
  • Environmental Microbiology
  • Hydrogen Peroxide / pharmacology*
  • Microbial Viability / drug effects*
  • Steam
  • Viruses / drug effects*


  • Disinfectants
  • Steam
  • Hydrogen Peroxide