Presence of heat shock protein 70 in secondary lymphoid tissue correlates with stroke prognosis

J Neuroimmunol. 2014 May 15;270(1-2):67-74. doi: 10.1016/j.jneuroim.2014.03.004. Epub 2014 Mar 12.

Abstract

Heat shock protein 70 (Hsp-70) can act as a danger signal and activate immune responses. We studied the presence of Hsp-70 in lymphoid tissue and plasma of acute stroke patients and asymptomatic controls free of neurological disease. Immunofluorescence, Western blotting, qRT-PCR and flow cytometry studies were performed. Plasma Hsp-70 concentration at day 7 was similar in patients and controls, whereas patients disclosed stronger immunoreactivity to Hsp-70 in lymphoid tissue than controls. Most Hsp-70+ cells were antigen presenting cells located in T cell zones. Stronger immunoreactivity to Hsp-70 was associated with smaller infarctions and better functional outcome.

Keywords: Acute stroke; Antigen presentation; Heat shock protein; Lymphoid tissue; Neuroimmunology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Biomarkers / analysis*
  • Biomarkers / metabolism
  • Blotting, Western
  • Female
  • Flow Cytometry
  • Fluorescent Antibody Technique
  • HSP70 Heat-Shock Proteins / analysis
  • HSP70 Heat-Shock Proteins / immunology
  • HSP70 Heat-Shock Proteins / metabolism*
  • Humans
  • Lymphoid Tissue / immunology
  • Lymphoid Tissue / metabolism*
  • Male
  • Prognosis
  • Real-Time Polymerase Chain Reaction
  • Recovery of Function / immunology
  • Stroke / immunology
  • Stroke / metabolism*

Substances

  • Biomarkers
  • HSP70 Heat-Shock Proteins