Autistic children display elevated urine levels of bovine casomorphin-7 immunoreactivity

Peptides. 2014 Jun:56:68-71. doi: 10.1016/j.peptides.2014.03.007. Epub 2014 Mar 20.


Elevated concentrations of circulating casomorphins (CM), the exogenous opioid peptides from milk casein, may contribute to the pathogenesis of autism in children. Because several mass spectrometry studies failed to detect casomorphins in autistic children, it was questioned whether these peptides can be detected in body fluids by mass spec. Here we demonstrated, using a novel high sensitivity ELISA method, that autistic children have significantly higher levels of urine CM-7 than control children. The severity of autistic symptoms correlated with concentrations of CM-7 in the urine. Because CMs interact with opioid and serotonin receptors, the known modulators of synaptogenesis, we suggest that chronic exposure to elevated levels of bovine CMs may impair early child development, setting the stage for autistic disorders.

Keywords: Autism; Bovine casomorphin; Opioid peptides; Urine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autistic Disorder / urine*
  • Child
  • Child, Preschool
  • Endorphins / urine*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Male
  • Opioid Peptides / urine


  • Endorphins
  • Opioid Peptides