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. 2014 Jul;26(7):1500-5.
doi: 10.1016/j.cellsig.2014.03.010. Epub 2014 Mar 18.

The miR-29b-Sirt1 Axis Regulates Self-Renewal of Mouse Embryonic Stem Cells in Response to Reactive Oxygen Species

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The miR-29b-Sirt1 Axis Regulates Self-Renewal of Mouse Embryonic Stem Cells in Response to Reactive Oxygen Species

Zengguang Xu et al. Cell Signal. .

Abstract

Endogenous reactive oxygen species (ROS) control is important for the maintenance of self-renewal of embryonic stem (ES) cells. Although miRNAs have been found to be critically involved in the regulation of the self-renewal, whether miRNAs can regulate the signaling axis to control ROS in ES cells is unclear. Here we show that miR-29b specifically regulates the self-renewal of mouse ES cells in response to ROS generated by antioxidant-free culture. Sirt1 is the direct target of miR-29b and can also make mES cells sensitive to ROS and regulate the self-renewal of mES cells during the response of ROS. We further found that Sirt1 could attenuate the miR-29b function in regulating mES cells' self-renewal in response to ROS. Our results determined that miR-29b-Sirt1 axis regulates self-renewal of mES cells in response to ROS.

Keywords: Mouse embryonic stem cell; Reactive oxygen species; Self-renewal; Sirt1; miR-29b.

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