Directed Axonal Outgrowth Using a Propagating Gradient of IGF-1

Adv Mater. 2014 Aug 6;26(29):4936-40. doi: 10.1002/adma.201305995. Epub 2014 Mar 24.

Abstract

The temporospatial regulation of axon outgrowth is useful for guiding de novo connectivity or re-connectivity of neurons in neurological injury or disease. Here we report the successful construction of a biocompatible guidance device, in which a linear propagation of IGF-1 gradient sequentially directs axon outgrowth. We observe the extensive in vitro axonal extension over 5 mm with a desired growth rate of ∼ 1 mm/day.

Keywords: axon guidance; controlled drug release; hydrogel; tissue engineering.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Axons / drug effects*
  • Axons / metabolism
  • Drug Carriers / chemistry
  • Drug Liberation
  • Humans
  • Insulin-Like Growth Factor I / chemistry
  • Insulin-Like Growth Factor I / pharmacology*
  • Lactic Acid / chemistry
  • Motor Neurons / cytology
  • Polyglycolic Acid / chemistry
  • Polylactic Acid-Polyglycolic Acid Copolymer

Substances

  • Drug Carriers
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polyglycolic Acid
  • Lactic Acid
  • Insulin-Like Growth Factor I