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. 2014 Jan;3(1):48-53.
doi: 10.4103/2278-330X.126524.

p53 and bcl2 Expression in Malignant and Premalignant Lesions of Uterine Cervix and Their Correlation With Human Papilloma Virus 16 and 18

Free PMC article

p53 and bcl2 Expression in Malignant and Premalignant Lesions of Uterine Cervix and Their Correlation With Human Papilloma Virus 16 and 18

Shailaja Shukla et al. South Asian J Cancer. .
Free PMC article


Background and objective: Persistent high risk human papilloma virus (HPV) infection is probably the best predictor of increased risk of cervical cancer, but expression of certain markers of cell proliferation and apoptosis have been studied. The present study was conducted to evaluate the expression of p53 and bcl2 in premalignant and malignant lesions of cervix and its correlation with HPV type 16 and 18.

Materials and methods: The study comprised of 35 cases (including 24 prospective cases and 11 retrospective cases) of premalignant and malignant lesions of the cervix. Slides were stained with Hematoxylin and Eosin and p53, bcl2 (immunohistochemistry), HPV 16 and HPV 18 (in situ hybridization).

Results: p53 positivity was seen in 8/19 (42.1%) cases of cervical intraepithelial neoplasia (CIN) and 8/16 (50%) cases of carcinoma cervix, the difference not significant statistically. The difference in bcl2 expression in CIN versus carcinoma cervix (84.21% vs. 43.75%) was statistically significant (P = 0.030). There was no significant difference between p53 and bcl2 expression and the stage and grade of the tumors. Seven out of 19 cases of CIN (36.84%) were positive for HPV 16/18 infection and 8/16 cases (50%) of carcinoma cervix were HPV positive (P = 0.628).

Conclusions: No significant association was found between HPV 16/18 infection and p53 and bcl2 expression in premalignant and malignant lesions of uterine cervix. Although, bcl2 staining showed a significant difference between CIN and carcinoma cervix, a larger case series is required to assess the association between HPV infection and overexpression of p53 and bcl2 proteins in these lesions.

Keywords: bcl2; cervix; human papilloma virus; p53.

Conflict of interest statement

Conflict of Interest: None declared.


Figure 1
Figure 1
(a) Squamous cell carcinoma showing p53 positivity as brown nuclear reactivity (p53 immunohistochemistry, × 400), (b) Adenocarcinoma showing intense p53 expression (p53 immunohistochemistry, ×400)
Figure 2
Figure 2
(a) Cervical intraepithelial neoplasia I showing bcl2 expression in the basal layer (bcl2 immunohistochemistry, ×100), (b) CIN II showing bcl 2 expression in the basal layer (bcl2 immunohistochemistry, ×400)
Figure 3
Figure 3
(a) Squamous cell carcinoma showing diffuse cytoplasmic bcl2 positivity (bcl2 immunohistochemistry, ×400), (b) Cytoplasmic bcl2 positivity in adenocarcinoma (bcl2 immunohistochemistry, ×400)
Figure 4
Figure 4
Cervical intraepithelial neoplasia II showing human papilloma virus 18 positivity seen as purple nuclear staining (in-situ Hybridization, HPV type 18)

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