Improved silver staining procedure for fast staining in PhastSystem Development Unit. I. Staining of sodium dodecyl sulfate gels

Electrophoresis. 1988 Jan;9(1):28-32. doi: 10.1002/elps.1150090106.

Abstract

A new modification of silver staining of proteins in sodium dodecyl sulfate polyacrylamide gels is adapted to automated staining in PhastSystem Development Unit. The use of a reduction step, after fixation, with thiosulfate in alcoholic sodium acetate buffer results in a considerable increase in sensitivity without the need for a recycling step. The detection limit is tenfold lower than in the silver staining procedure recommended so far for PhastSystem and corresponds to 0.05-0.1 ng protein per band. Total staining time with the new procedure is 75 min.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkylation
  • Electrophoresis, Polyacrylamide Gel / instrumentation
  • Electrophoresis, Polyacrylamide Gel / methods*
  • Oxidation-Reduction
  • Proteins / analysis
  • Silver*
  • Sodium Dodecyl Sulfate
  • Staining and Labeling*

Substances

  • Proteins
  • Sodium Dodecyl Sulfate
  • Silver