Improved Staining of Proteins in Polyacrylamide Gels Including Isoelectric Focusing Gels With Clear Background at Nanogram Sensitivity Using Coomassie Brilliant Blue G-250 and R-250

Electrophoresis. 1988 Jun;9(6):255-62. doi: 10.1002/elps.1150090603.

Abstract

An improved procedure for staining of proteins following separation in polyacrylamide gels is described which utilizes the colloidal properties of Coomassie Brilliant Blue G-250 and R-250. The new method is based on addition of 20% v/v methanol and higher concentrations of ammonium sulfate to the staining solution previously described. The method combines the advantage of much shorter staining time with high sensitivity, a clear background not requiring destaining, stepwise staining, and stable fixation after staining. The method has been applied to staining of polyacrylamide gels after sodium dodecyl sulfate-electrophoresis and isoelectric focusing in carrier ampholyte-generated pH gradients.

MeSH terms

  • Acrylic Resins
  • Densitometry
  • Electrophoresis, Polyacrylamide Gel
  • Gels
  • Isoelectric Focusing
  • Light
  • Proteins / analysis*
  • Rosaniline Dyes
  • Scattering, Radiation
  • Staining and Labeling

Substances

  • Acrylic Resins
  • Gels
  • Proteins
  • Rosaniline Dyes
  • polyacrylamide gels
  • Coomassie brilliant blue R
  • coomassie Brilliant Blue