APUM5, encoding a Pumilio RNA binding protein, negatively regulates abiotic stress responsive gene expression

BMC Plant Biol. 2014 Mar 25:14:75. doi: 10.1186/1471-2229-14-75.

Abstract

Background: A mutant screening was carried out previously to look for new genes related to the Cucumber mosaic virus infection response in Arabidopsis. A Pumilio RNA binding protein-coding gene, Arabidopsis Pumilio RNA binding protein 5 (APUM5), was obtained from this screening.

Results: APUM5 transcriptional profiling was carried out using a bioinformatics tool. We found that APUM5 was associated with both biotic and abiotic stress responses. However, bacterial and fungal pathogen infection susceptibility was not changed in APUM5 transgenic plants compared to that in wild type plants although APUM5 expression was induced upon pathogen infection. In contrast, APUM5 was involved in the abiotic stress response. 35S-APUM5 transgenic plants showed hypersensitive phenotypes under salt and drought stresses during germination, primary root elongation at the seedling stage, and at the vegetative stage in soil. We also showed that some abiotic stress-responsive genes were negatively regulated in 35S-APUM5 transgenic plants. The APUM5-Pumilio homology domain (PHD) protein bound to the 3' untranslated region (UTR) of the abiotic stress-responsive genes which contained putative Pumilio RNA binding motifs at the 3' UTR.

Conclusions: These results suggest that APUM5 may be a new post-transcriptional regulator of the abiotic stress response by direct binding of target genes 3' UTRs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions / genetics
  • Abscisic Acid / pharmacology
  • Adaptation, Physiological / drug effects
  • Adaptation, Physiological / genetics
  • Alternaria / drug effects
  • Alternaria / physiology
  • Arabidopsis / genetics*
  • Arabidopsis / immunology
  • Arabidopsis / microbiology
  • Arabidopsis / physiology*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Base Sequence
  • Disease Resistance / drug effects
  • Disease Resistance / genetics
  • Disease Resistance / immunology
  • Down-Regulation / drug effects
  • Down-Regulation / genetics
  • Droughts
  • Gene Expression Profiling
  • Gene Expression Regulation, Plant* / drug effects
  • Genes, Plant
  • Genes, Reporter
  • Germination / drug effects
  • Germination / genetics
  • Glucuronidase / metabolism
  • Mannitol / pharmacology
  • Molecular Sequence Data
  • Organ Specificity / drug effects
  • Organ Specificity / genetics
  • Plant Diseases / genetics
  • Plant Diseases / immunology
  • Plant Diseases / microbiology
  • Plant Roots / drug effects
  • Plant Roots / growth & development
  • Plants, Genetically Modified
  • Promoter Regions, Genetic / genetics
  • Pseudomonas syringae / drug effects
  • Pseudomonas syringae / physiology
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism*
  • Sodium Chloride / pharmacology
  • Stress, Physiological / drug effects
  • Stress, Physiological / genetics*
  • Transcription, Genetic / drug effects

Substances

  • 3' Untranslated Regions
  • APUM5 protein, Arabidopsis
  • Arabidopsis Proteins
  • RNA-Binding Proteins
  • Mannitol
  • Sodium Chloride
  • Abscisic Acid
  • Glucuronidase