Biomanufacture of human platelets for transfusion: Rationale and approaches

Exp Hematol. 2014 May;42(5):332-46. doi: 10.1016/j.exphem.2014.02.002. Epub 2014 Mar 22.

Abstract

Platelets for transfusion obtained from volunteer blood donors are a limited resource. Given the increased range of donor restrictions to prevent transmission of disease and the decline in volunteer blood donors, there is a diminishing supply of blood for transfusion. Production of mature blood cells from hematopoietic stem cells via large-scale manufacture is an alternative way of meeting transfusion demands. In this review, we provide a detailed outline of the challenges and opportunities for the biomanufacture of platelets. We describe the scale required for platelet biomanufacture to deliver sufficient cells for transfusion, provide a brief outline of the current understanding of megakaryopoiesis and thrombogenesis, and highlight how the current understanding impacts the design of culture systems and bioreactors for producing platelets.

Publication types

  • Review

MeSH terms

  • Blood Platelets*
  • Cell Culture Techniques / methods*
  • Cells, Cultured
  • Hematopoietic Stem Cells*
  • Humans
  • Thrombopoiesis*
  • Transforming Growth Factor beta*

Substances

  • Transforming Growth Factor beta