Opsin- And S-antigen-like Immunoreactions in Photoreceptors of the Tree Shrew Retina

Invest Ophthalmol Vis Sci. 1989 Mar;30(3):530-5.

Abstract

In the tree shrew retina individual rod and cone photoreceptors can be readily identified and quantified because their perikarya are arranged in a single layer. This retina is therefore an ideal system for testing the specificity of photoreceptor-directed antibodies. Here we describe the staining properties of polyclonal antibodies against (rhod)opsin and retinal S-antigen in the tree shrew retina. The (rhod)opsin antibody exclusively and completely labelled the rod population. The antibody against S-antigen also labelled all rods and, in addition, a regularly arrayed subpopulation of cones, which we argue to be the blue-sensitive cones. In the context of our findings, the labelling of pinealocytes with these antibodies is discussed.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / immunology
  • Antigens / immunology*
  • Arrestin
  • Eye Proteins / immunology*
  • Female
  • Immunohistochemistry
  • Male
  • Photoreceptor Cells / immunology*
  • Retina / cytology
  • Retina / immunology*
  • Retina / ultrastructure
  • Rhodopsin / immunology
  • Rod Opsins
  • Staining and Labeling
  • Tupaiidae

Substances

  • Antibodies
  • Antigens
  • Arrestin
  • Eye Proteins
  • Rod Opsins
  • Rhodopsin