miR-99a and -99b inhibit cervical cancer cell proliferation and invasion by targeting mTOR signaling pathway

Med Oncol. 2014 May;31(5):934. doi: 10.1007/s12032-014-0934-3. Epub 2014 Mar 26.

Abstract

MicroRNAs were demonstrated to play an important role in the regulation of gene expression. Here, we showed that miR-99a and -99b (miR-99a/b) were down-regulated in human cervical cancer patient tissues and were negatively related with lymphatic metastasis. In addition, overexpression of miR-99a/b inhibited cell growth and invasion, whereas suppression of miR-99a/b yielded the reverse phenotype. Dual luciferase report assay revealed that mTOR was identified as a novel target gene of both miR-99a and -99b. Altogether, these results suggested that miR-99a/b directly and negatively regulated mTOR expression in cervical cancer cells, and enforced the importance of miR-99a/b and their targets in the malignant phenotypes of cervical carcinogenesis.

MeSH terms

  • Blotting, Western
  • Carcinoma, Squamous Cell / genetics
  • Carcinoma, Squamous Cell / metabolism
  • Carcinoma, Squamous Cell / pathology*
  • Cell Movement*
  • Cell Proliferation*
  • Female
  • Humans
  • Luciferases / metabolism
  • MicroRNAs / antagonists & inhibitors
  • MicroRNAs / genetics*
  • Middle Aged
  • Neoplasm Invasiveness
  • RNA, Messenger / genetics
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction
  • TOR Serine-Threonine Kinases / antagonists & inhibitors
  • TOR Serine-Threonine Kinases / genetics
  • TOR Serine-Threonine Kinases / metabolism*
  • Tumor Cells, Cultured
  • Uterine Cervical Neoplasms / genetics
  • Uterine Cervical Neoplasms / metabolism
  • Uterine Cervical Neoplasms / pathology*

Substances

  • MIRN99 microRNA, human
  • MicroRNAs
  • RNA, Messenger
  • Luciferases
  • MTOR protein, human
  • TOR Serine-Threonine Kinases