Isolation of functionally active T cell receptor gamma delta-bearing lymphocytes from human peripheral blood

J Immunol Methods. 1989 Mar 31;118(2):251-5. doi: 10.1016/0022-1759(89)90013-6.

Abstract

We have developed a simple method for isolation of functionally active T cell receptor (TCR)-gamma delta positive cells from human peripheral blood, using immunomagnetic separation techniques. After culture with feeder cells and interleukin-2, cells thus isolated showed a CD3+ TCR-alpha beta- phenotype, and stained with antibodies against the TCR-gamma delta complex. The TCR-gamma delta+ cells were functionally active, responding with DNA synthesis when stimulated via their CD3 and CD2 molecules in concert, or when interleukin-2 was added.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antibodies, Monoclonal
  • Cell Line
  • Cell Separation* / methods
  • Flow Cytometry
  • Humans
  • Leukocyte Count
  • Magnetics
  • Microspheres
  • Phenotype
  • Receptors, Antigen, T-Cell* / analysis
  • Staining and Labeling
  • T-Lymphocytes / analysis
  • T-Lymphocytes / classification*
  • T-Lymphocytes / physiology

Substances

  • Antibodies, Monoclonal
  • Receptors, Antigen, T-Cell