Herpes simplex virus growth, preparation, and assay

Methods Mol Biol. 2014;1144:19-29. doi: 10.1007/978-1-4939-0428-0_2.

Abstract

In order to study the biology of herpes simplex virus or to use it as a vector in gene therapy, it is necessary to grow the virus and to prepare virus stocks. Many different protocols are available from different research groups working with herpes simplex virus type 1 or 2 (HSV-1 or HSV-2). This chapter describes the procedures used in our laboratory.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Genetic Therapy
  • Genetic Vectors
  • Herpesvirus 1, Human / genetics*
  • Herpesvirus 1, Human / growth & development
  • Herpesvirus 1, Human / pathogenicity
  • Herpesvirus 2, Human / genetics*
  • Herpesvirus 2, Human / growth & development
  • Herpesvirus 2, Human / pathogenicity
  • Humans
  • Molecular Biology / methods*