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Identification of Carotenoids From the Extremely Halophilic Archaeon Haloarcula Japonica

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Identification of Carotenoids From the Extremely Halophilic Archaeon Haloarcula Japonica

Rie Yatsunami et al. Front Microbiol.

Abstract

The carotenoids produced by extremely halophilic archaeon Haloarcula japonica were extracted and identified by their chemical, chromatographic, and spectroscopic characteristics (UV-Vis and mass spectrometry). The composition (mol%) was 68.1% bacterioruberin, 22.5% monoanhydrobacterioruberin, 9.3% bisanhydrobacterioruberin, <0.1% isopentenyldehydrorhodopin, and trace amounts of lycopene and phytoene. The in vitro scavenging capacity of a carotenoid, bacterioruberin, extracted from Haloarcula japonica cells against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals was evaluated. The antioxidant capacity of bacterioruberin was much higher than that of β -carotene.

Keywords: C50 carotenoid; Haloarcula japonica; antioxidant capacity; bacterioruberin; extremely halophilic archaeon.

Figures

FIGURE 1
FIGURE 1
Elution profile of pigments extracted from Haloarcula japonica. The HPLC system consisted of a reversed-phase μBondapack C18 column. Absorbance at 490 nm is shown. Peak identification is revealed in Table 1. St: internal standard of ethyl β-apo-8′-carotenoate.
FIGURE 2
FIGURE 2
Structure of carotenoids produced by Haloarcula japonica.
FIGURE 3
FIGURE 3
Radical scavenging activities of BR and β-carotene against DPPH radicals. White bars, β-carotene; Black bars, BR.

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