Structure of the yeast mitochondrial large ribosomal subunit

Science. 2014 Mar 28;343(6178):1485-1489. doi: 10.1126/science.1249410.


Mitochondria have specialized ribosomes that have diverged from their bacterial and cytoplasmic counterparts. We have solved the structure of the yeast mitoribosomal large subunit using single-particle cryo-electron microscopy. The resolution of 3.2 angstroms enabled a nearly complete atomic model to be built de novo and refined, including 39 proteins, 13 of which are unique to mitochondria, as well as expansion segments of mitoribosomal RNA. The structure reveals a new exit tunnel path and architecture, unique elements of the E site, and a putative membrane docking site.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cryoelectron Microscopy
  • Mitochondria / ultrastructure*
  • Mitochondrial Proteins / chemistry
  • Nucleic Acid Conformation
  • RNA, Ribosomal / chemistry
  • RNA, Transfer / chemistry
  • Ribosome Subunits, Large, Eukaryotic / chemistry
  • Ribosome Subunits, Large, Eukaryotic / ultrastructure*
  • Saccharomyces cerevisiae / ultrastructure*


  • Mitochondrial Proteins
  • RNA, Ribosomal
  • RNA, Transfer

Associated data

  • PDB/3J6B