Abstract
A set of 2-chloro-4-nitrophenyl glucosamino-/xylosaminosides were synthesized and assessed as potential substrates in the context of glycosyltransferase-catalyzed formation of the corresponding UDP/TDP-α-D-glucosamino-/xylosaminosugars and in single-vessel model transglycosylation reactions. This study highlights a robust platform for aminosugar nucleotide synthesis and reveals OleD Loki to be a proficient catalyst for U/TDP-aminosugar synthesis and utilization
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Amines / chemistry
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Amines / metabolism*
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Carbohydrate Metabolism*
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Carbohydrates / chemistry
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Catalysis
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Glucosides / chemistry
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Glucosides / metabolism
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Glycosyltransferases / genetics
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Glycosyltransferases / metabolism*
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Nitrophenols / chemistry
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Nitrophenols / metabolism
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Nucleotides / chemistry
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Nucleotides / metabolism*
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Protein Engineering
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Streptomyces antibioticus / enzymology*
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Streptomyces antibioticus / genetics
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Substrate Specificity
Substances
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Amines
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Carbohydrates
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Glucosides
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Nitrophenols
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Nucleotides
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4-nitrophenyl
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Glycosyltransferases