New anti-GD2 monoclonal antibodies produced from gamma-interferon-treated neuroblastoma cells

Int J Cancer. 1989 Apr 15;43(4):665-71. doi: 10.1002/ijc.2910430421.

Abstract

Three monoclonal antibodies (IgG2) have been produced from hybridomas obtained by fusion of murine myeloma cells and spleen cells of mice hyperimmunized with gamma-interferon-treated neuroblastoma cells. The 3 MAbs, 7A4, 2A6 and IG8, detected an antigen present on neuroblastoma tumors and cell lines, but also on some neuro-ectoderm-derived tissues and cells. All 3 clones were shown to react with an epitope of the di-sialo-ganglioside GD2 molecules highly expressed by some neuro-ectoderm-derived tumors, mainly neuroblastoma. Whereas MAb IG8 specificity was restricted to GD2 and its o-acylated form, MAb 2A6 and 7A4 were also able to detect GD3 at high concentration of antibody as shown by TLC analysis and immunodetection. The 3 MAbs were able to lyse 100% neuroblastoma cells in the presence of rabbit or human complement. Direct binding assays with 125I-labelled MAbs showed that MAb 7A4 might be a good candidate for in vivo immunolocalization experiments. The high proportion of anti-GD2 MAbs obtained by our fusion and the increased binding of anti-GD2 MAbs on gamma-IFN-treated neuroblastoma cells suggests a modulation of the exposure and an increase in the immunogenicity of GD2 induced by gamma-IFN.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / analysis
  • Antibodies, Monoclonal / biosynthesis*
  • Antibodies, Monoclonal / isolation & purification
  • Antigens, Neoplasm / analysis
  • Binding Sites, Antibody / drug effects
  • Cell Line
  • Complement System Proteins / immunology
  • Cytotoxicity, Immunologic
  • Epitopes / analysis
  • Epitopes / immunology
  • Gangliosides / immunology*
  • Gangliosides / isolation & purification
  • Humans
  • Hybridomas / drug effects
  • Hybridomas / immunology
  • Immunization / methods
  • Immunoglobulin Isotypes / analysis
  • Interferon-gamma / pharmacology*
  • Mice
  • Neuroblastoma / immunology*

Substances

  • Antibodies, Monoclonal
  • Antigens, Neoplasm
  • Epitopes
  • Gangliosides
  • Immunoglobulin Isotypes
  • ganglioside, GD2
  • Interferon-gamma
  • Complement System Proteins