Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors

J Immunol. 1989 Apr 15;142(8):2736-42.


Research on the specificities, functions, and maturation of T cells would be greatly aided by a collection of monoclonal antibodies which distinguishes different types of TCR. With this end in mind hamsters were immunized and tested for production of pan-reactive anti-mouse alpha beta TCR antibodies. In this report we describe the properties and uses of a mAb, H57-597, produced from one of these animals. The mAb reacts with surface receptors on all alpha beta TCR-bearing cells and does not react with receptors on gamma delta+ T cells. In an immobilized form, this antibody can directly activate T cells bearing alpha beta TCR. It can be used in immunoprecipitation reactions to precipitate receptor from the appropriate cell types. In combination with anti-CD3, the antibody can be used in cytofluorographic analyses to measure numbers of CD3+, alpha beta+, and CD3+, gamma delta+ cells in the thymus and periphery.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antigens, Differentiation, T-Lymphocyte / analysis
  • CD3 Complex
  • Cricetinae
  • Cricetulus
  • Epitopes / analysis
  • Mice
  • Receptors, Antigen, T-Cell / analysis*
  • Receptors, Antigen, T-Cell / immunology
  • Receptors, Antigen, T-Cell, alpha-beta
  • T-Lymphocytes / immunology


  • Antibodies, Monoclonal
  • Antigens, Differentiation, T-Lymphocyte
  • CD3 Complex
  • Epitopes
  • Receptors, Antigen, T-Cell
  • Receptors, Antigen, T-Cell, alpha-beta