A simple, sensitive method of analyzing bacterial ribosomal DNA polymorphism

N Picard-Pasquier; M Ouagued; B Picard; P Goullet; R Krishnamoorthy

doi:10.1002/elps.1150100306

A simple, sensitive method of analyzing bacterial ribosomal DNA polymorphism

Electrophoresis. 1989 Mar;10(3):186-9. doi: 10.1002/elps.1150100306.

Authors

N Picard-Pasquier¹, M Ouagued, B Picard, P Goullet, R Krishnamoorthy

Affiliation

¹ Institut de Pathologie Moléculaire, Institut National de la Santé et de la Recherche Médicale, Paris, France.

PMID: 2468482
DOI: 10.1002/elps.1150100306

Abstract

A rapid DNA extraction procedure and random primer labelling of Escherichia coli ribosomal RNA with cloned reverse transcriptase have been used to establish a simple and highly sensitive method for studying ribosomal DNA polymorphism in bacteria. Examples of inter- and intraspecies differentiation of bacteria are given and potential applications in bacterial epidemiology and taxonomy are discussed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acinetobacter / genetics
Autoradiography
Bacteria / genetics*
DNA, Bacterial / analysis*
DNA, Ribosomal / analysis*
Enterobacteriaceae / genetics
Ethidium
Methods
Polymorphism, Genetic*
Polymorphism, Restriction Fragment Length*
RNA Probes
RNA-Directed DNA Polymerase
Species Specificity

Substances

DNA, Bacterial
DNA, Ribosomal
RNA Probes
RNA-Directed DNA Polymerase
Ethidium