A simple, sensitive method of analyzing bacterial ribosomal DNA polymorphism

Electrophoresis. 1989 Mar;10(3):186-9. doi: 10.1002/elps.1150100306.

Abstract

A rapid DNA extraction procedure and random primer labelling of Escherichia coli ribosomal RNA with cloned reverse transcriptase have been used to establish a simple and highly sensitive method for studying ribosomal DNA polymorphism in bacteria. Examples of inter- and intraspecies differentiation of bacteria are given and potential applications in bacterial epidemiology and taxonomy are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acinetobacter / genetics
  • Autoradiography
  • Bacteria / genetics*
  • DNA, Bacterial / analysis*
  • DNA, Ribosomal / analysis*
  • Enterobacteriaceae / genetics
  • Ethidium
  • Methods
  • Polymorphism, Genetic*
  • Polymorphism, Restriction Fragment Length*
  • RNA Probes
  • RNA-Directed DNA Polymerase
  • Species Specificity

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA Probes
  • RNA-Directed DNA Polymerase
  • Ethidium