Neural crest development and craniofacial morphogenesis is coordinated by nitric oxide and histone acetylation

Chem Biol. 2014 Apr 24;21(4):488-501. doi: 10.1016/j.chembiol.2014.02.013. Epub 2014 Mar 27.


Cranial neural crest (CNC) cells are patterned and coalesce to facial prominences that undergo convergence and extension to generate the craniofacial form. We applied a chemical genetics approach to identify pathways that regulate craniofacial development during embryogenesis. Treatment with the nitric oxide synthase inhibitor 1-(2-[trifluoromethyl] phenyl) imidazole (TRIM) abrogated first pharyngeal arch structures and induced ectopic ceratobranchial formation. TRIM promoted a progenitor CNC fate and inhibited chondrogenic differentiation, which were mediated through impaired nitric oxide (NO) production without appreciable effect on global protein S-nitrosylation. Instead, TRIM perturbed hox gene patterning and caused histone hypoacetylation. Rescue of TRIM phenotype was achieved with overexpression of histone acetyltransferase kat6a, inhibition of histone deacetylase, and complementary NO. These studies demonstrate that NO signaling and histone acetylation are coordinated mechanisms that regulate CNC patterning, differentiation, and convergence during craniofacial morphogenesis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation / drug effects
  • Animals
  • Cell Differentiation / drug effects
  • Chondrogenesis / drug effects
  • Histones / metabolism*
  • Imidazoles / pharmacology
  • Morphogenesis* / drug effects
  • Neural Crest / drug effects
  • Neural Crest / embryology*
  • Neural Crest / metabolism*
  • Nitric Oxide / metabolism*
  • Signal Transduction / drug effects
  • Zebrafish / embryology*
  • Zebrafish / metabolism*


  • Histones
  • Imidazoles
  • 1-(2-trifluoromethylphenyl)imidazole
  • Nitric Oxide