MALDI imaging mass spectrometry: spatial molecular analysis to enable a new age of discovery

J Proteomics. 2014 Jul 31;107:71-82. doi: 10.1016/j.jprot.2014.03.021. Epub 2014 Mar 29.

Abstract

Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) combines the sensitivity and selectivity of mass spectrometry with spatial analysis to provide a new dimension for histological analyses to provide unbiased visualization of the arrangement of biomolecules in tissue. As such, MALDI IMS has the capability to become a powerful new molecular technology for the biological and clinical sciences. In this review, we briefly describe several applications of MALDI IMS covering a range of molecular weights, from drugs to proteins. Current limitations and challenges are discussed along with recent developments to address these issues. This article is part of a Special Issue entitled: 20years of Proteomics in memory of Viatliano Pallini. Guest Editors: Luca Bini, Juan J. Calvete, Natacha Turck, Denis Hochstrasser and Jean-Charles Sanchez.

Keywords: MALDI imaging mass spectrometry.

Publication types

  • Historical Article
  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Drug Discovery
  • History, 20th Century
  • History, 21st Century
  • Humans
  • Proteins
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / history
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

  • Proteins