Recent advances in enrichment and separation strategies for mass spectrometry-based phosphoproteomics

Electrophoresis. 2014 Dec;35(24):3418-29. doi: 10.1002/elps.201400017. Epub 2014 Jun 16.


Due to the significance of protein phosphorylation in various biological processes and signaling events, new analytical techniques for enhanced phosphoproteomics have been rapidly introduced in the recent years. The combinatorial use of the phospho-specific enrichment techniques and prefractionation methods prior to MS analysis enable comprehensive profiling of the phosphoproteome and facilitate deciphering the critical roles that phosphorylation plays in signaling pathways in various biological systems. This review places special emphasis on the recent five-year (2009-2013) advances for enrichment and separation techniques that have been utilized for phosphopeptides prior to MS analysis.

Keywords: Enrichment; Mass spectrometry; Phosphopeptides; Phosphoproteomics/Separation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Humans
  • Mass Spectrometry / methods*
  • Phosphopeptides / analysis
  • Phosphopeptides / chemistry*
  • Phosphopeptides / isolation & purification
  • Phosphoproteins / analysis
  • Phosphoproteins / chemistry*
  • Phosphoproteins / isolation & purification
  • Proteomics / methods*


  • Phosphopeptides
  • Phosphoproteins