Detection of enterovirus genome sequence from diarrheal feces of goat

Virus Genes. 2014 Jun;48(3):550-2. doi: 10.1007/s11262-014-1057-9. Epub 2014 Apr 2.


Goat diarrheal feces were subjected to metagenome analysis by the next-generation sequencing. Nucleotide sequences with homology to enteroviruses were obtained. Primers for RT-PCR were designed based on the nucleotide sequence of these sequences at the 5'-untranslated region, and we determined 563 bp nucleotide sequences that showed homology to bovine-like and ovine enteroviruses (77-87 %). We named the virus detected in this study goat enterovirus G1 (GEV-G1). In the phylogenetic analysis, GEV-G1 belonged to a cluster containing ovine enteroviruses. To our knowledge, this is the first report on nucleotide sequences of an enterovirus infecting Japanese goats.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cattle
  • Diarrhea / veterinary*
  • Diarrhea / virology
  • Enterovirus / classification
  • Enterovirus / genetics
  • Enterovirus / isolation & purification*
  • Feces / virology*
  • Genome, Viral*
  • Goat Diseases / virology*
  • Goats
  • Molecular Sequence Data
  • Phylogeny
  • Sheep

Associated data

  • GENBANK/AB857843