Alcian blue staining of proteoglycans in polyacrylamide gels using the "critical electrolyte concentration" approach

R S Wall; T J Gyi

doi:10.1016/0003-2697(88)90392-2

Alcian blue staining of proteoglycans in polyacrylamide gels using the "critical electrolyte concentration" approach

Anal Biochem. 1988 Nov 15;175(1):298-9. doi: 10.1016/0003-2697(88)90392-2.

Authors

R S Wall¹, T J Gyi

Affiliation

¹ Open University, Oxford Research Unit, United Kingdom.

PMID: 2469353
DOI: 10.1016/0003-2697(88)90392-2

Abstract

A method is described here for Alcian blue staining of proteoglycans in polyacrylamide gels; this is illustrated using extracts obtained from bovine corneal stroma. Other available methods for visualization of proteoglycans can produce nonspecific staining and frequently a high background in the gel; with a "critical electrolyte concentration" approach, specific staining against a clear background can be obtained.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Alcian Blue
Electrophoresis, Polyacrylamide Gel
Proteoglycans / isolation & purification*
Staining and Labeling / methods*

Substances

Proteoglycans
Alcian Blue

Grants and funding

EY05405/EY/NEI NIH HHS/United States