A B72.3 second-generation-monoclonal antibody (CC49) defines the mucin-carried carbohydrate epitope Gal beta(1-3) [NeuAc alpha(2-6)]GalNAc

Biol Chem Hoppe Seyler. 1989 Jan;370(1):21-6. doi: 10.1515/bchm3.1989.370.1.21.

Abstract

Mucus glycoproteins from bovine seminal plasma were demonstrated to express the MAbs CC49- and B72.3-defined epitopes on TAG 72 antigen. Inhibition studies with reductively cleaved mucin O-glycans from bovine seminal plasma and submaxillary glands revealed that CC49 binds specifically to a core-type sialyl-oligosaccharide alditol (fraction 2c), which could be defined with regard to its primary structure by FAB- and EI-mass spectrometry combined with methylation analysis: (formula; see text) Structurally related alditols NeuAc alpha(2-6)-GalNAc-ol, Gal beta(1-3)GalNAc-ol, NeuAc alpha(2-3)-Gal beta(1-3) [NeuAc alpha(2-6)] GalNAc-ol, GlcNAc beta(1-3) [NeuAc alpha(2-6)] GalNAc-ol or NeuAc alpha(2-3) Gal beta(1-3)GalNAc-ol were not inhibitory.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antigens, Neoplasm / immunology
  • Carbohydrates / immunology
  • Cattle
  • Epitopes / analysis
  • Epitopes / immunology*
  • Epitopes / isolation & purification
  • Glycoproteins / immunology
  • Humans
  • Mucins / analysis
  • Mucins / immunology*
  • Prostatic Secretory Proteins*
  • Proteins / analysis
  • Seminal Plasma Proteins
  • Submandibular Gland / analysis
  • Sugar Alcohols / immunology
  • Trisaccharides

Substances

  • Antibodies, Monoclonal
  • Antigens, Neoplasm
  • Carbohydrates
  • Epitopes
  • Glycoproteins
  • Mucins
  • Prostatic Secretory Proteins
  • Proteins
  • Seminal Plasma Proteins
  • Sugar Alcohols
  • Trisaccharides
  • beta-microseminoprotein
  • tumor-associated antigen 72