Characterization and antibacterial properties of N-halamine-derivatized cross-linked polymethacrylamide nanoparticles

Biomaterials. 2014 Jun;35(19):5079-87. doi: 10.1016/j.biomaterials.2014.02.056. Epub 2014 Mar 30.


N-halamine-derivatized cross-linked polymethacrylamide nanoparticles with sizes ranging between 18 ± 2.0 and 460 ± 60 nm were prepared via surfactant-free dispersion co-polymerization of methacrylamide (MAA) and the cross-linking monomer N,N-methylenebisacrylamide (MBAA) in an aqueous continuous phase, followed by a chlorination process using sodium hypochlorite. The effect of various polymerization parameters (monomer concentration, initiator type and concentration, polymerization duration, polymerization temperature, and the weight ratio [MBAA]/[MAA]) on the size and size distribution of the produced cross-linked P(MAA-MBAA) nanoparticles was elucidated. The effect of various chlorination parameters (hypochlorite concentration, chlorination period and temperature) on the bound oxidative chlorine atom (Cl) content of the P(MAA-MBAA) nanoparticles was also investigated. The bactericidal activity of these chloramine-derivatized nanoparticles was tested against two common bacterial pathogens (Escherichia coli and Staphylococcus aureus), and they were found to be highly potent. Furthermore, these nanoparticles also exerted their antimicrobial activity against multi-drug resistant (MDR) bacteria, further demonstrating their efficacy.

Keywords: Antibacterial properties; N-halamine nanoparticles; Polyamide nanoparticles; Polymethacrylamide nanoparticles.

MeSH terms

  • Anti-Bacterial Agents / chemistry*
  • Anti-Bacterial Agents / pharmacology
  • Microbial Sensitivity Tests
  • Nanoparticles / chemistry*
  • Nylons / chemistry
  • Staphylococcus aureus / drug effects


  • Anti-Bacterial Agents
  • Nylons