Surfactant protein SP-D modulates activity of immune cells: proteomic profiling of its interaction with eosinophilic cells

Expert Rev Proteomics. 2014 Jun;11(3):355-69. doi: 10.1586/14789450.2014.897612. Epub 2014 Apr 3.

Abstract

Surfactant protein D (SP-D), a C-type lectin, is known to protect against lung infection, allergy and inflammation. Its recombinant truncated form comprising homotrimeric neck and CRD region (rhSP-D) has been shown to bring down specific IgE levels, eosinophilia and restore Th2-Th1 homeostasis in murine models of lung hypersensitivity. SP-D knockout mice show intrinsic hypereosinophilia and airway hyper-responsiveness that can be alleviated by rhSP-D. The rhSP-D can bind activated eosinophils, inhibit chemotaxis and degranulation, and selectively induce oxidative burst and apoptosis in sensitized eosinophils. A global proteomics study of rhSP-D-treated eosinophilic cell line AML14.3D10 identified large-scale molecular changes associated with oxidative burst, cell stress and survival-related proteins potentially responsible for apoptosis induction. The data also suggested an involvement of RNA binding- and RNA splicing-related proteins. Thus, the proteomics approach yielded a catalog of differentially expressed proteins that may be protein signatures defining mechanisms of SP-D-mediated maintenance of homeostasis during allergy.

Keywords: SP-D; allergy; apoptosis; eosinophilia; heterogeneous nuclear ribonucleoproteins; p53 pathway; proteomics; surfactant.

Publication types

  • Review

MeSH terms

  • Adaptive Immunity
  • Animals
  • Eosinophilia / immunology
  • Eosinophilia / metabolism
  • Eosinophils / metabolism*
  • Humans
  • Hypersensitivity / immunology
  • Hypersensitivity / metabolism
  • Immunity, Innate
  • Leukemia, Myeloid, Acute / immunology
  • Leukemia, Myeloid, Acute / metabolism
  • Proteome / metabolism*
  • Pulmonary Surfactant-Associated Protein D / metabolism*
  • Recombinant Proteins / pharmacology

Substances

  • Proteome
  • Pulmonary Surfactant-Associated Protein D
  • Recombinant Proteins