Acute toxicity test of a natural iron chelator and an antioxidant, extracted from Triticum aestivum Linn. (wheat grass)

Nat Prod Res. 2014;28(17):1379-82. doi: 10.1080/14786419.2014.901321. Epub 2014 Apr 4.


Triticum aestivum (wheat grass) is widely used in traditional medicine to treat various diseases. Previously the purified compounds and crude extract of T. aestivum were established to have iron chelation potency and antioxidant activity. So it is necessary to evaluate the toxic properties of any compound isolated from plant extract to prevent any untoward side effects. The aim of this study was to determine the acute oral toxicity level of our purified compounds, i.e. mugineic acids and methylpheophorbide a., and crude extract of T. aestivum, on Swiss albino mice at dosage of 2000 mg/kg for a period of 14 days using the organisation for economic co-operation and development guidelines 423. There was no mortality. No change in behavioural pattern, clinical signs, body weight and blood biochemistry profile were observed. Kidney and liver showed normal histo-pathological architecture. Hence, the oral administration of compounds and extract of T. aestivum did not produce any significant toxic effect on mice. Thus we may conclude that the extract can be utilised for pharmaceutical formulations as iron chelator and antioxidant agent for various diseases.

Keywords: Triticum aestivum; acute oral toxicity; methylpheophorbide a; mice modelling; mugineic acids; wheat grass.

MeSH terms

  • Animals
  • Antioxidants / analysis*
  • Antioxidants / isolation & purification*
  • Iron Chelating Agents / analysis*
  • Kidney / metabolism
  • Liver / metabolism
  • Male
  • Mice
  • Toxicity Tests, Acute
  • Triticum / chemistry*


  • Antioxidants
  • Iron Chelating Agents