Glycopolymer functionalization of engineered spider silk protein-based materials for improved cell adhesion

Macromol Biosci. 2014 Jul;14(7):936-42. doi: 10.1002/mabi.201400020. Epub 2014 Apr 2.


Silk protein-based materials are promising biomaterials for application as tissue scaffolds, due to their processability, biocompatibility, and biodegradability. The preparation of films composed of an engineered spider silk protein (eADF4(C16)) and their functionalization with glycopolymers are described. The glycopolymers bind proteins found in the extracellular matrix, providing a biomimetic coating on the films that improves cell adhesion to the surfaces of engineered spider silk films. Such silk-based materials have potential as coatings for degradable implantable devices.

Keywords: biodegradable; biomaterials; biomimetics; cell adhesion; coatings; glycopolymers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Biocompatible Materials / pharmacology*
  • Biopolymers / chemistry
  • Biopolymers / pharmacology*
  • Cell Adhesion / drug effects
  • Fibroblasts / cytology*
  • Fibroblasts / drug effects
  • Mice
  • Molecular Sequence Data
  • Polysaccharides / chemical synthesis
  • Polysaccharides / chemistry
  • Polysaccharides / pharmacology*
  • Protein Binding / drug effects
  • Protein Engineering*
  • Rats
  • Silk / chemistry
  • Silk / pharmacology*
  • Spiders / chemistry*
  • Surface Properties


  • Biocompatible Materials
  • Biopolymers
  • Polysaccharides
  • Silk