Illuminaio: An Open Source IDAT Parsing Tool for Illumina Microarrays

F1000Res. 2013 Dec 4;2:264. doi: 10.12688/f1000research.2-264.v1. eCollection 2013.

Abstract

The IDAT file format is used to store BeadArray data from the myriad of genomewide profiling platforms on offer from Illumina Inc. This proprietary format is output directly from the scanner and stores summary intensities for each probe-type on an array in a compact manner. A lack of open source tools to process IDAT files has hampered their uptake by the research community beyond the standard step of using the vendor's software to extract the data they contain in a human readable text format. To fill this void, we have developed the illuminaio package that parses IDAT files from any BeadArray platform, including the decryption of files from Illumina's gene expression arrays. illuminaio provides the first open-source package for this task, and will promote wider uptake of the IDAT format as a standard for sharing Illumina BeadArray data in public databases, in the same way that the CEL file serves as the standard for the Affymetrix platform.

Grant support

This work was supported by the European Community’s Seventh Framework Programme under grant agreement No. 305626 (Project RADIANT) (MLS), NHMRC Project grant 1050661, Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS (MER) and Grant Number R01 GM103552 from the National Institute of General Medical Sciences, National Institutes of Health (KDH).