Abstract
The group I intron from Tetrahymena catalyzes phosphodiester transfer reactions on various RNA substrates. A modified RNA substrate with a phosphorothioate group in one stereoisomeric form at the site of reaction was synthesized in order to determine the stereochemical course of an RNA-catalyzed reaction. The reaction product was digested with a stereospecific nuclease to determine the configuration of the product phosphorothioate. The reaction occurs with inversion of configuration at phosphorus, implying an in-line pathway for the reaction.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Catalysis
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DNA-Directed RNA Polymerases / metabolism
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Exons
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Guanosine / metabolism
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Introns
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Molecular Conformation
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Oligonucleotides / metabolism
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Phosphorus
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RNA / chemical synthesis
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RNA / metabolism
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RNA Precursors / metabolism
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RNA Splicing
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RNA, Catalytic
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RNA, Ribosomal / metabolism*
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Ribonucleases / metabolism
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Structure-Activity Relationship
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T-Phages / enzymology
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Templates, Genetic
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Tetrahymena / genetics*
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Thionucleotides / metabolism
Substances
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Oligonucleotides
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RNA Precursors
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RNA, Catalytic
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RNA, Ribosomal
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Thionucleotides
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Guanosine
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Phosphorus
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RNA
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DNA-Directed RNA Polymerases
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Ribonucleases