Site-specific Cross-Linking of Collagen Peptides by Lysyl Advanced Glycation Endproducts

Chem Commun (Camb). 2014 May 18;50(38):4944-6. doi: 10.1039/c4cc02003k.

Abstract

Cross-linking of proteins by advanced glycation endproducts (AGEs) causes a host of pathological conditions but their exact roles are unknown. Cross-linking lysyl AGEs were synthesized and incorporated into two types of collagen peptides. The utility of these cross-linked peptides for biochemical investigations was demonstrated by proteolysis studies and circular dichroism.

MeSH terms

  • Chromatography, High Pressure Liquid
  • Circular Dichroism
  • Collagen / chemistry*
  • Collagen / metabolism
  • Dimerization
  • Glycation End Products, Advanced / chemistry*
  • Glycation End Products, Advanced / metabolism
  • Glyoxal / chemistry
  • Lysine / chemistry
  • Pyruvaldehyde / chemistry
  • Trypsin / metabolism

Substances

  • Glycation End Products, Advanced
  • Glyoxal
  • Pyruvaldehyde
  • Collagen
  • Trypsin
  • Lysine