The promoter elements of the mouse myelin basic protein gene function efficiently in NG108-15 neuronal/glial cells

Gene. 1989 Jan 30;75(1):31-8. doi: 10.1016/0378-1119(89)90380-6.


We measured transiently-expressed beta-galactosidase activity by introducing the mouse myelin basic protein (MBP)-lacZ chimeric gene (MBP-lacZ) into the NG108-15 neuronal/glial hybrid cell line. Deletion studies of the promoter region of the MBP gene showed that the promoter region between -1318 bp and -254 bp might contain sequences that repress MBP promoter activity. Fine deletion analysis using BAL 31 exonuclease revealed sequences between bp -208 and -140, -139 and -118, and -89 and -75 which were critical for promoter activity in NG 108-15 cells. DNaseI footprinting analysis revealed a cellular factor(s) that bind to the promoter region between bp -127 and -106 with NG108-15 whole cell extracts. The SV40 promoter was activated by insertion of the sequences around the region protected in footprinting experiments, in a manner independent of its orientation in NG108-15 cells. This protected region is thought to be one of the critical cis-acting DNA elements for efficient transcription.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cell Line
  • Chimera
  • Chromosome Deletion
  • Deoxyribonuclease I / metabolism
  • Hybrid Cells
  • Mice
  • Molecular Sequence Data
  • Myelin Basic Protein / genetics*
  • Promoter Regions, Genetic*
  • Simian virus 40 / genetics
  • Transcription, Genetic
  • Transfection
  • beta-Galactosidase / metabolism


  • Myelin Basic Protein
  • Deoxyribonuclease I
  • beta-Galactosidase

Associated data

  • GENBANK/M24410