Epidermal Wnt/β-catenin signaling regulates adipocyte differentiation via secretion of adipogenic factors

Proc Natl Acad Sci U S A. 2014 Apr 15;111(15):E1501-9. doi: 10.1073/pnas.1312880111. Epub 2014 Mar 31.


It has long been recognized that the hair follicle growth cycle and oscillation in the thickness of the underlying adipocyte layer are synchronized. Although factors secreted by adipocytes are known to regulate the hair growth cycle, it is unclear whether the epidermis can regulate adipogenesis. We show that inhibition of epidermal Wnt/β-catenin signaling reduced adipocyte differentiation in developing and adult mouse dermis. Conversely, ectopic activation of epidermal Wnt signaling promoted adipocyte differentiation and hair growth. When the Wnt pathway was activated in the embryonic epidermis, there was a dramatic and premature increase in adipocytes in the absence of hair follicle formation, demonstrating that Wnt activation, rather than mature hair follicles, is required for adipocyte generation. Epidermal and dermal gene expression profiling identified keratinocyte-derived adipogenic factors that are induced by β-catenin activation. Wnt/β-catenin signaling-dependent secreted factors from keratinocytes promoted adipocyte differentiation in vitro, and we identified ligands for the bone morphogenetic protein and insulin pathways as proadipogenic factors. Our results indicate epidermal Wnt/β-catenin as a critical initiator of a signaling cascade that induces adipogenesis and highlight the role of epidermal Wnt signaling in synchronizing adipocyte differentiation with the hair growth cycle.

Keywords: niche cross-talk; skin; stem cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / physiology*
  • Animals
  • Azo Compounds
  • Cell Differentiation / physiology*
  • Cluster Analysis
  • Epidermal Cells
  • Epidermis / physiology*
  • Flow Cytometry
  • Hair Follicle / growth & development*
  • Humans
  • Keratinocytes / metabolism
  • Mice
  • Wnt Signaling Pathway / physiology*
  • beta Catenin / metabolism*


  • Azo Compounds
  • beta Catenin
  • oil red O