Deregulated expression of circadian clock genes in gastric cancer

BMC Gastroenterol. 2014 Apr 6;14:67. doi: 10.1186/1471-230X-14-67.

Abstract

Background: Gastric cancer (GC), an aggressive malignant tumor of the alimentary tract, is a leading cause of cancer-related death. Circadian rhythm exhibits a 24-hour variation in physiological processes and behavior, such as hormone levels, metabolism, gene expression, sleep and wakefulness, and appetite. Disruption of circadian rhythm has been associated with various cancers, including chronic myeloid leukemia, head and neck squamous cell carcinoma, hepatocellular carcinoma, endometrial carcinoma, and breast cancer. However, the expression of circadian clock genes in GC remains unexplored.

Methods: In this study, the expression profiles of eight circadian clock genes (PER1, PER2, PER3, CRY1, CRY2, CKIϵ, CLOCK, and BMAL1) of cancerous and noncancerous tissues from 29 GC patients were investigated using real-time quantitative reverse-transcriptase polymerase chain reaction and validated through immunohistochemical analysis.

Results: We found that PER2 was significantly up-regulated in cancer tissues (p < 0.005). Up-regulated CRY1 expression was significantly correlated with more advanced stages (stage III and IV) (p < 0.05).

Conclusions: Our results suggest deregulated expressions of circadian clock genes exist in GC and circadian rhythm disturbance may be associated with the development of GC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ARNTL Transcription Factors / genetics*
  • Adenocarcinoma / genetics*
  • Aged
  • Aged, 80 and over
  • CLOCK Proteins / genetics*
  • Case-Control Studies
  • Cryptochromes / genetics*
  • Cyclin-Dependent Kinase Inhibitor Proteins / genetics*
  • Female
  • Gene Expression Regulation, Neoplastic / genetics*
  • Humans
  • Male
  • Middle Aged
  • Period Circadian Proteins / genetics*
  • Stomach Neoplasms / genetics*

Substances

  • ARNTL Transcription Factors
  • ARNTL protein, human
  • Cryptochromes
  • Cyclin-Dependent Kinase Inhibitor Proteins
  • Period Circadian Proteins
  • CLOCK Proteins