Characterization of an antiproliferative exopolysaccharide (LHEPS-2) from Lactobacillus helveticus MB2-1

Carbohydr Polym. 2014 May 25;105:334-40. doi: 10.1016/j.carbpol.2014.01.093. Epub 2014 Feb 5.

Abstract

In this study, we reported that three purified exopolysaccharides fractions (LHEPS-1, LHEPS-2 and LHEPS-3) and crude LHEPS from the Lactobacillus helveticus MB2-1 inhibited the proliferation potential of human gastric cancer BGC-823 cells. We found that all the LHEPS fractions and crude LHEPS exerted concentration and time dependent inhibitory activities in vitro on BGC-823 cells. LHEPS-2 exhibited higher antiproliferative activity against BGC-823 cells in vitro than LHEPS-1, LHEPS-3 and crude LHEPS. With the consideration of the outstanding antiproliferative effect of LHEPS-2 on BGC-823 cells, more fine structural characterization of LHEPS-2 was further identified. The structure of LHEPS-2 was elucidated by methylated analysis, GC-MS, (1)H NMR and (13)C NMR spectroscopy, together with two-dimensional (2D) COSY, TOCSY, HSQC, HMBC and NOESY spectroscopy. Results showed that the LHEPS-2 was consisted of the following repeating units:

Keywords: Antiproliferative activity; Exopolysaccharides; Lactobacillus helveticus MB2-1; Nuclear magnetic resonance spectroscopy; Structural characterization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / chemistry*
  • Antineoplastic Agents / isolation & purification
  • Antineoplastic Agents / pharmacology*
  • Carbohydrate Sequence
  • Cell Line, Tumor
  • Cell Proliferation / drug effects*
  • Humans
  • Lactobacillus helveticus / chemistry*
  • Molecular Sequence Data
  • Polysaccharides, Bacterial / chemistry*
  • Polysaccharides, Bacterial / isolation & purification
  • Polysaccharides, Bacterial / pharmacology*
  • Stomach Neoplasms / drug therapy

Substances

  • Antineoplastic Agents
  • Polysaccharides, Bacterial