Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen

Nat Commun. 2014 Apr 7;5:3591. doi: 10.1038/ncomms4591.

Abstract

Cancer cells secrete small membranous extracellular vesicles (EVs) into their microenvironment and circulation. Although their potential as cancer biomarkers has been promising, the identification and quantification of EVs in clinical samples remains challenging. Here we describe a sensitive and rapid analytical technique for profiling circulating EVs directly from blood samples of patients with colorectal cancer. EVs are captured by two types of antibodies and are detected by photosensitizer-beads, which enables us to detect cancer-derived EVs without a purification step. We also show that circulating EVs can be used for detection of colorectal cancer using the antigen CD147, which is embedded in cancer-linked EVs. This work describes a new liquid biopsy technique to sensitively detect disease-specific circulating EVs and provides perspectives in translational medicine from the standpoint of diagnosis and therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Basigin / metabolism
  • Biomarkers, Tumor / blood*
  • Biomarkers, Tumor / metabolism
  • Case-Control Studies
  • Colorectal Neoplasms / blood*
  • Colorectal Neoplasms / metabolism
  • Enzyme-Linked Immunosorbent Assay
  • Extracellular Space
  • HT29 Cells
  • Humans
  • Immunoblotting
  • Secretory Vesicles / metabolism*
  • Tetraspanin 28 / metabolism
  • Tetraspanin 29 / metabolism
  • Tetraspanin 30 / metabolism

Substances

  • BSG protein, human
  • Biomarkers, Tumor
  • CD63 protein, human
  • CD81 protein, human
  • CD9 protein, human
  • Tetraspanin 28
  • Tetraspanin 29
  • Tetraspanin 30
  • Basigin