Quantitative phosphoproteomics analysis of nitric oxide-responsive phosphoproteins in cotton leaf

PLoS One. 2014 Apr 8;9(4):e94261. doi: 10.1371/journal.pone.0094261. eCollection 2014.

Abstract

Knowledge of phosphorylation events and their regulation is crucial to understanding the functional biology of plant proteins, but very little is currently known about nitric oxide-responsive phosphorylation in plants. Here, we report the first large-scale, quantitative phosphoproteome analysis of cotton (Gossypium hirsutum) treated with sodium nitroprusside (nitric oxide donor) by utilizing the isobaric tag for relative and absolute quantitation (iTRAQ) method. A total of 1315 unique phosphopeptides, spanning 1528 non-redundant phosphorylation sites, were detected from 1020 cotton phosphoproteins. Among them, 183 phosphopeptides corresponding to 167 phosphoproteins were found to be differentially phosphorylated in response to sodium nitroprusside. Several of the phosphorylation sites that we identified, including RQxS, DSxE, TxxxxSP and SPxT, have not, to our knowledge, been reported to be protein kinase sites in other species. The phosphoproteins identified are involved in a wide range of cellular processes, including signal transduction, RNA metabolism, intracellular transport and so on. This study reveals unique features of the cotton phosphoproteome and provides new insight into the biochemical pathways that are regulated by nitric oxide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Gossypium / metabolism*
  • Nitric Oxide / metabolism*
  • Phosphopeptides / metabolism
  • Phosphoproteins / chemistry
  • Phosphoproteins / genetics
  • Phosphoproteins / metabolism*
  • Phosphorylation
  • Plant Leaves / metabolism*
  • Plant Proteins / chemistry
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Proteome / metabolism*
  • Proteomics*

Substances

  • Phosphopeptides
  • Phosphoproteins
  • Plant Proteins
  • Proteome
  • Nitric Oxide

Grants and funding

This research was sponsored by the National Basic Research Program of China (973 Program; 2010CB126006) and supported by the State Key Laboratory of Cotton Biology Open Fund (CB2013A01). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.