Selective modulation of GABAergic tonic current by dopamine in the nucleus accumbens of alcohol-dependent rats

J Neurophysiol. 2014 Jul 1;112(1):51-60. doi: 10.1152/jn.00564.2013. Epub 2014 Apr 9.

Abstract

The nucleus accumbens (NAcc) is a key structure of the mesolimbic dopaminergic reward system and plays an important role in mediating alcohol-seeking behaviors. Alterations in glutamatergic and GABAergic signaling were recently demonstrated in the NAcc of rats after chronic intermittent ethanol (CIE) treatment, a model of alcohol dependence. Here we studied dopamine (DA) modulation of GABAergic signaling and how this modulation might be altered by CIE treatment. We show that the tonic current (I(tonic)) mediated by extrasynaptic γ-aminobutyric acid type A receptors (GABA(A)Rs) of medium spiny neurons (MSNs) in the NAcc core is differentially modulated by DA at concentrations in the range of those measured in vivo (0.01-1 μM), without affecting the postsynaptic kinetics of miniature inhibitory postsynaptic currents (mIPSCs). Use of selective D1 receptor (D1R) and D2 receptor (D2R) ligands revealed that I(tonic) potentiation by DA (10 nM) is mediated by D1Rs while I(tonic) depression by DA (0.03-1 μM) is mediated by D2Rs in the same MSNs. Addition of guanosine 5'-O-(2-thiodiphosphate) (GDPβS) to the recording pipettes eliminated I(tonic) decrease by the selective D2R agonist quinpirole (5 nM), leaving intact the quinpirole effect on mIPSC frequency. Recordings from CIE and vehicle control (CIV) MSNs during application of D1R agonist (SKF 38393, 100 nM) or D2R agonist (quinpirole, 2 nM) revealed that SKF 38393 potentiated I(tonic) to the same extent, while quinpirole reduced I(tonic) to a similar extent, in both groups of rats. Our data suggest that the selective modulatory effects of DA on I(tonic) are unaltered by CIE treatment and withdrawal.

Keywords: ethanol; neuroadaptation; tonic current; ventral striatum; withdrawal.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine / pharmacology
  • Alcoholism / metabolism*
  • Alcoholism / physiopathology
  • Animals
  • Dopamine / pharmacology*
  • Dopamine Agonists / pharmacology*
  • Guanosine Diphosphate / analogs & derivatives
  • Guanosine Diphosphate / pharmacology
  • Inhibitory Postsynaptic Potentials*
  • Male
  • Miniature Postsynaptic Potentials*
  • Neurons / drug effects
  • Neurons / metabolism
  • Neurons / physiology*
  • Nucleus Accumbens / cytology
  • Nucleus Accumbens / metabolism*
  • Nucleus Accumbens / physiopathology
  • Quinpirole / pharmacology
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Dopamine D1 / agonists
  • Receptors, Dopamine D2 / agonists
  • Receptors, GABA-A / metabolism
  • Thionucleotides / pharmacology

Substances

  • Dopamine Agonists
  • Receptors, Dopamine D1
  • Receptors, Dopamine D2
  • Receptors, GABA-A
  • Thionucleotides
  • Guanosine Diphosphate
  • Quinpirole
  • 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine
  • guanosine 5'-O-(2-thiodiphosphate)
  • Dopamine