Adventures in time and space: splicing efficiency and RNA polymerase II elongation rate

RNA Biol. 2014;11(4):313-9. doi: 10.4161/rna.28646. Epub 2014 Apr 2.

Abstract

Control of pre-mRNA splicing is a critical part of the eukaryotic gene expression process. Extensive evidence indicates that transcription and splicing are spatiotemporally coordinated and that most splicing events occur co-transcriptionally. A kinetic coupling model has been proposed in metazoans to describe how changing RNA Polymerase II (RNAPII) elongation rate can impact which alternative splice sites are used. In Saccharomyces cerevisiae, in which most spliced genes have only a single intron and splice sites adhere to a strong consensus sequence, we recently observed that splicing efficiency was sensitive to mutations in RNAPII that increase or decrease its elongation rate. Our data revealed that RNAPII speed and splicing efficiency are generally anti-correlated: at many genes, increased elongation rate caused decreased splicing efficiency, while decreased elongation rate increased splicing efficiency. An improved splicing phenotype was also observed upon deletion of SUB1, a condition in which elongation rate is slowed. We discuss these data in the context of a growing field and expand the kinetic coupling model to apply to both alternative splicing and splicing efficiency.

Keywords: RNA polymerase II; elongation rate; kinetic coupling; splicing; splicing efficiency.

Publication types

  • Review

MeSH terms

  • Humans
  • Kinetics
  • RNA Polymerase II / metabolism*
  • RNA Splicing / physiology*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Transcription Elongation, Genetic*
  • Transcription, Genetic

Substances

  • RNA Polymerase II