Isolation and characterization of a functional murine interferon alpha gene which is not expressed in fibroblasts upon virus induction

J Gen Virol. 1989 Jun;70 ( Pt 6):1381-9. doi: 10.1099/0022-1317-70-6-1381.

Abstract

A mouse genomic segment containing three new members of the murine interferon alpha (MuIFN-alpha) gene family was isolated from a fibroblastic cosmid library. A 4 kb EcoRI fragment contained a new MuIFN-alpha gene named MuIFN-alpha 8. The nucleotide sequence of the coding and flanking regions of this gene showed a high level of homology to those of known members of the MuIFN-alpha family. Transient expression of the MuIFN-alpha 8 gene in COS cells and oocyte translation of in vitro transcripts both led to a biologically active protein. The antiviral activity was neutralized by monoclonal and polyclonal MuIFN-alpha antibodies. Although the 5' flanking sequence shows features characteristic of an IFN regulatory region, the MuIFN-alpha 8 gene is not expressed in murine fibroblasts treated with Newcastle disease virus or poly(I).poly(C).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cell Line
  • Cosmids
  • Fibroblasts
  • Gene Expression Regulation*
  • Genes*
  • Interferon Type I / biosynthesis
  • Interferon Type I / genetics*
  • Interferon Type I / immunology
  • Mice
  • Molecular Sequence Data
  • Newcastle disease virus / physiology
  • Poly I-C / pharmacology
  • Protein Biosynthesis
  • RNA / genetics
  • Regulatory Sequences, Nucleic Acid
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic
  • Vesicular stomatitis Indiana virus / physiology

Substances

  • Interferon Type I
  • RNA
  • Poly I-C