Generation of colonic IgA-secreting cells in the caecal patch

Nat Commun. 2014 Apr 10;5:3704. doi: 10.1038/ncomms4704.

Abstract

Gut-associated lymphoid tissues are responsible for the generation of IgA-secreting cells. However, the function of the caecal patch, a lymphoid tissue in the appendix, remains unknown. Here we analyse the role of the caecal patch using germ-free mice colonized with intestinal bacteria after appendectomy. Appendectomized mice show delayed accumulation of IgA(+) cells in the large intestine, but not the small intestine, after colonization. Decreased colonic IgA(+) cells correlate with altered faecal microbiota composition. Experiments using photoconvertible Kaede-expressing mice or adoptive transfer show that the caecal patch IgA(+) cells migrate to the large and small intestines, whereas Peyer's patch cells are preferentially recruited to the small intestine. IgA(+) cells in the caecal patch express higher levels of CCR10. Dendritic cells in the caecal patch, but not Peyer's patches, induce CCR10 on cocultured B cells. Thus, the caecal patch is a major site for generation of IgA-secreting cells that migrate to the large intestine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adoptive Transfer
  • Animals
  • Appendix / cytology*
  • Appendix / immunology*
  • Base Sequence
  • Cell Movement / immunology*
  • Colon / cytology*
  • DNA Primers / genetics
  • Dendritic Cells / immunology
  • Feces / microbiology
  • Flow Cytometry
  • Immunoglobulin A / immunology*
  • Immunoglobulin A / metabolism
  • Immunohistochemistry
  • Luminescent Proteins / metabolism
  • Lymphoid Tissue / immunology*
  • Lymphoid Tissue / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Microbiota / genetics*
  • Molecular Sequence Data
  • Peyer's Patches / cytology
  • RNA, Ribosomal, 16S / genetics
  • Real-Time Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Statistics, Nonparametric

Substances

  • DNA Primers
  • Immunoglobulin A
  • Kaede protein, Trachyphyllia geoffroyi
  • Luminescent Proteins
  • RNA, Ribosomal, 16S