Monoclonal antibody that defines the prostate specific antigen

Prostate. 1989;14(3):237-49. doi: 10.1002/pros.2990140305.

Abstract

A monoclonal antibody to a protein with a single band in the 30-kD region was obtained from fusion of Balb/C mouse spleen cells immunized with epithelial fractions of BPH, with the myeloma cell line P3 x 63 Ag8 6.5.3 by standard procedures. This antigen was characterized in immunobinding studies with various cellular and target antigens and in immunoperoxidase staining.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antibodies, Monoclonal / isolation & purification
  • Antigens, Neoplasm / analysis
  • Antigens, Neoplasm / immunology*
  • Biomarkers, Tumor / analysis
  • Biomarkers, Tumor / immunology*
  • Blotting, Western
  • Chromatography, Affinity
  • Fluorescent Antibody Technique
  • Humans
  • Hybridomas
  • Immunoblotting
  • Immunodiffusion
  • Immunoenzyme Techniques
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Prostate / immunology*
  • Prostate-Specific Antigen
  • Prostatic Neoplasms / immunology*
  • Radioimmunoassay

Substances

  • Antibodies, Monoclonal
  • Antigens, Neoplasm
  • Biomarkers, Tumor
  • Prostate-Specific Antigen